Laser Microdissection Workshop for Examiners Agenda Day 1

Time
Topic
Instructor(s)
8:30 – 9:00 a.m. Welcome and Introduction
  1. Instructors
  2. Students
  3. Facilities
  4. Agenda Review

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Sanders, Wojtkiewicz,
Raley
9:00 – 10:00 a.m. Introduction to LMD technology
  1. Basic microscopy
    1. Components
    2. imaging
    3. Fluorescence
    4. DIC
  2. Laser dissection
    1. Basic theory
    2. History of LMD microscopy
    3. Other LMD technologies
Wojtkiewicz
10:00 – 10:15 a.m. Break  
10:15 - 10:30 a.m. Basic cell identification: sperm and epithelial cells

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Raley
10:30 – 10:45 a.m. Lab Practical Overview Sanders
10:45 11:00 a.m. WET/CHEM LAB - Preparing smears on PEN slides for LMD

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Sanders
11:00 – 11:15 a.m. LMD LAB Care and Basics of LMD 6000 Lee
11:15 – 11:30 a.m. WET/CHEM LAB – Staining Slides Sanders
11:30 a.m. – 12:00 p.m. LMD LAB - Demo of Leica LMD 6000: Using the
microscope
Sanders, Raley
12:00 – 1:00 p.m. Lunch  
1:00 –1:15 p.m. LMD dissection of sperm cells from a mixture Sanders
1:15 – 2:00 p.m. LMD LAB - Demo of Cell Dissection Sanders, Raley
2:30 – 2:45 p.m. Break  
2:15 – 3:15 p.m.
LMD LAB - LMD exercises Recovery of cell samples Groups 1 & 2
WET/CHEM LAB - Fluorescence Sample Prep. - Groups 3 & 4
Sanders, Raley
Wojtkiewicz
3:15 – 4:15 p.m.
LMD LAB - LMD exercises – Recovery of cell samples Groups 3 & 4
WET/CHEM LAB - Fluorescence Sample Prep. - Groups 1 & 2
Sanders, Raley
Wojtkiewicz
4:15 – 4:30 p.m. Discussions

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S

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