Laser Microdissection Workshop for Examiners Agenda – Day 2

Time	Topic	Instructor(s)
8:30 – 8:45	Q&A Review of protocols performed by NFSTC staff Forensic LMD Literature	Sanders
8:45 – 9:45	Research Studies Technical Obstacles Histology Study Isolation Study DNA Yield Study Mixture Study Low Copy Number Study Flash \| Quicktime \| WMV	Sanders
9:45 – 10:00	Break
10:00 – 10:30	Research Studies cont… Comparative Study with Preferential Lysis Case Studies Flash \| Quicktime \| WMV	Sanders
10:30 – 11:00	Advanced LMD Forensic Applications Fluorescent Sperm Identification Chromosome Paint for Diploid Cells Flash \| Quicktime \| WMV	Wojtkiewicz
11:00 – 12:00	LMD LAB Fluorescence AVC Software Module Advanced Operations	Wojtkiewicz Lee
12:00 – 1:00	Lunch
1:00 – 1:45	LMD Microscopy Research and Experience Why LMD for NLCL Casework Elimination of Manual Extraction Reproducibility Sensitivity Troubleshooting Single Amplification/Optimization Absence of Sperm LMD: Streamlined, Novel Process Casework Examples Flash \| Quicktime \| WMV	Raley
	Quick Break
1:00 – 1:45	Research on Chromosome Paint of Diploid Cells Cell Preparation for Chromosome Paint Male/Female Diploid Cells Mixtures Non-probative case results Flash \| Quicktime \| WMV	Wojtkiewicz
2:30 – 2:45	Break
2:45 – 3:15	Review DNA Data from LMD samples	Sanders
3:15 – 4:00	Novel Processing Plan for LMD Analysis of Sexual Assault Evidence Flash \| Quicktime \| WMV	Wojtkiewicz
4:00 – 4:30	Discussions and Closing