Day 1
Welcome and Introductions
8:00 a.m.–8:15 a.m.
- Purpose of the workshop
- Instructors
- Facilities
Overview of SNP Analysis
8:15 a.m.–9:00 a.m.
- History of SNPs in forensic testing
- Types of SNPs suitable for forensic typing
- SNP applications in other fields of science
Genetics of SNP Markers
9:00 a.m.–9:45 a.m.
- Selection of SNP loci
- Population structure & expectations
Break
Genplex™: A CE-based SNP detection platform
10:00 a.m.–11:30 a.m.
- Genplex™ process
- Amplification and electrophoresis parameters
- Data interpretation
Lunch
Overview of Ibis™ ESI-MS
12:30 p.m.–2:00 p.m.
- Introduction to mass spectrometry Presentation (PDF 2.4MB)
- Introduction to the Ibis™ platforms and base composition analysis Presentation (PDF 7.3MB)
Break
Lab tour, group assignments, intro to technologies
2:15 p.m.–5:15 p.m.
- Group 1 – GenPlex™ (hands-on) Presentation (PDF 764kB)
- Group 2 – Ibis™ T5000™/PLEX-ID™ (lecture)
- Workflow overview Presentation (PDF 912kB)
- Instrumentation overview Presentation (PDF 588kB)
- Overview of assays:
- SNPs Presentation (PDF 2.5MB)
- mtDNA Presentation (PDF 1.7MB)
- STRs Presentation (PDF 1.4MB)
- Y-STRs Presentation (PDF 948kB)
- Software overview Presentation (PDF 4.8MB)