Laser Microdissection Workshop for Examiners Agenda – Day 2
Time
Topic
Instructor(s)
8:30 – 8:45 a.m.
  1. Q&A
  2. Review of protocols performed by NFSTC staff
  3. Forensic LMD Literature
 Sanders
8:45 – 9:45 a.m. Research Studies
  1. Technical Obstacles
  2. Histology Study
  3. Isolation Study
  4. DNA Yield Study
  5. Mixture Study
  6. Low Copy Number Study

Watch Video		 Sanders
9:45 – 10:00 a.m. Break  
10:00 – 10:30 a.m. Research Studies cont…
  1. Comparative Study with Preferential Lysis
  2. Case Studies

Watch Video

Sanders

 
10:30 – 11:00 a.m. Advanced LMD Forensic Applications
  1. Fluorescent Sperm Identification
  2. Chromosome Paint for Diploid Cells

Watch Video		 Wojtkiewicz
11:00 a.m. – 12:00 p.m. LMD LAB
  1. Fluorescence
  2. AVC Software Module
  3. Advanced Operations
 Wojtkiewicz
Lee
12:00 – 1:00 p.m. Lunch  
1:00 – 1:45 p.m. LMD Microscopy Research and Experience
  1. Why LMD for NLCL Casework
  2. Elimination of Manual Extraction
  3. Reproducibility
  4. Sensitivity
  5. Troubleshooting
  6. Single Amplification/Optimization
  7. Absence of Sperm
  8. LMD: Streamlined, Novel Process
  9. Casework Examples

Watch Video		 Raley
  Quick Break  
1:45 – 2:30 p.m. Research on Chromosome Paint of Diploid Cells
  1. Cell Preparation for Chromosome Paint
  2. Male/Female Diploid Cells Mixtures
  3. Non-probative case results

Watch Video		 Wojtkiewicz
2:30 – 2:45 p.m. Break  
2:45 – 3:15 p.m. Review DNA Data from LMD samples Sanders
3:15 – 4:00 p.m. Novel Processing Plan for LMD Analysis of Sexual Assault Evidence
Watch Video		 Wojtkiewicz
4:00 – 4:30 p.m. Discussions and Closing  

Go to Day 1

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