One Day Agenda

Welcome and Introductions

8:30 a.m.–8:45 a.m.

• Purpose of the workshop
• Instructors
• Facilities
• NIJ website overview
• Agenda review

Introduction to Biological Mass Spectrometry

8:45 a.m.–9:15 a.m.

• Brief history
• Components
• Applications

Presentation (PDF 4MB)

High Throughput Analysis of Amplified Nucleic Acids with Mass Spectrometry: The Ibis™ Platform

9:15 a.m.–9:45 a.m.

• Applications
• Ibis™ PLEX-ID™

Presentation (PDF 12.3MB)

Break

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Overview of the Ibis™ STR Assay

10:00 a.m.–10:45 a.m.

• Assay format
• Comparison to traditional STR analysis
• Polymorphic STR alleles
• Sensitivity
• Reproducibility

Presentation (PDF 3.2MB)

Overview of the Ibis™ Y-STR, mtDNA and SNP Assays

10:45 a.m.–11:30 a.m.

• Ibis™ Y-STR assay format
• Ibis™ mtDNA assay format and information content relative to sequencing
• Ibis™ SNP assay format

Presentation (PDF 19MB)

DNA Mixture Detection, Analysis, and Interpretation Using the Ibis™ Assays

10:30 a.m.–12:00 p.m.

Presentation (PDF 3.2MB)

Lunch

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Laboratory Tour and Demonstration of the Ibis™ PLEX-ID™

1:00 p.m.–2:00 p.m.

Presentation (PDF 420kB)

Break

Enhanced Resolution and Statistical Power Through SNP Distributions Within the Short Tandem Repeats

2:15 p.m.–2:45 p.m.

• Nature and structure of STR loci
• SNP distributions with the STR repeat structure
• Population and statistical implications of expanded allele definitions
• Assumptions and implications for database searching

Presentation (PDF 2.3MB)

Future Directions of Biological Mass Spec Analyses

2:45 p.m.–3:15 p.m.

Break

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Technology Transition Topics Discussion Session and Closing Remarks

3:30 p.m.–4:30 p.m.