The Global Forensic and Justice Center at Florida International University is dedicated to supporting the justice community through innovative research, programs, evaluation of the latest technologies, forensic science education and laboratory quality reviews. Our goal is for all stakeholders to receive and provide the highest level of forensic science services.

As part of this commitment, we are making available the nearly 30-year archive of training material, symposia, poster presentations, and technical evaluations completed by our founding component, the National Forensic Science Technology Center (NFSTC).

Physical Separation of Forensic Mixtures Technology Transition Workshop, 2010

Sponsored by Investigative and Forensic Sciences Division, Office of Science and Technology, National Institute of Justice, U.S. Department of Justice

Downloadable Files:

Welcome and introductions

  1. Purpose of the workshop
  2. Instructors
  3. Facilities
  4. Safety and laboratory protocols
  5. Agenda review
  6. Brief overview of laser microdissection (LM) and fluorescent in situ hybridization (FISH) processes

Presentation (PDF 484kB)

Introduction to LM

  1. Applications
    • Mixtures of cells with same morphology
    • Mixtures of cells with different morphology
  2. Instrumentation
    • Laser microdissection (LM)
    • Laser capture microdissection (LCM)

Presentation (PDF 836kB)

Introduction to FISH

  1. General overview
  2. Procedure
    • CEP probes
  3. Downstream processing
    • Laser microdissection
    • Extraction
    • Amplification
  4. Applications
    • Advantages / limitations

Presentation (PDF 760kB)

LM Methods

Presentation (PDF 1.1MB)

Role of LM and FISH in the Forensic Lab

Presentation (PDF 224kB)

Available Instrument Specifics

Leica (PDF 1.4MB) | MMI (PDF 1MB) | Zeiss (PDF 1.4MB)

Review of Laboratory Results

  1. Analysis of extracted LM samples
  2. Discussion of results
  3. LM instruments open session

Results Obtained By Workshop Participants (PDF 876kB)

Closing Discussion

Presentation (PDF 288kB)

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Global Forensic and Justice Center

11200 SW 8th Street
Miami, FL 33199

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