The Global Forensic and Justice Center at Florida International University is dedicated to supporting the justice community through innovative research, programs, evaluation of the latest technologies, forensic science education and laboratory quality reviews. Our goal is for all stakeholders to receive and provide the highest level of forensic science services.
As part of this commitment, we are making available the nearly 30-year archive of training material, symposia, poster presentations, and technical evaluations completed by our founding component, the National Forensic Science Technology Center (NFSTC).
Physical Separation of Forensic Mixtures Technology Transition Workshop, 2010
Sponsored by Investigative and Forensic Sciences Division, Office of Science and Technology, National Institute of Justice, U.S. Department of Justice
Downloadable Files:
Welcome and introductions
- Purpose of the workshop
- Instructors
- Facilities
- Safety and laboratory protocols
- Agenda review
- Brief overview of laser microdissection (LM) and fluorescent in situ hybridization (FISH) processes
Introduction to LM
- Applications
- Mixtures of cells with same morphology
- Mixtures of cells with different morphology
- Instrumentation
- Laser microdissection (LM)
- Laser capture microdissection (LCM)
Introduction to FISH
- General overview
- Procedure
- CEP probes
- Downstream processing
- Laser microdissection
- Extraction
- Amplification
- Applications
- Advantages / limitations
LM Methods
- Using the Carl Zeiss PALM MicroBeam System
- Target cell amounts
- Extraction methods
- Advantages / limitations
Role of LM and FISH in the Forensic Lab
- When should LM processing be used?
- Implementation
- Cost-benefit analysis
Available Instrument Specifics
- Overviews of commercially available LM instruments
Leica (PDF 1.4MB) | MMI (PDF 1MB) | Zeiss (PDF 1.4MB)
Review of Laboratory Results
- Analysis of extracted LM samples
- Discussion of results
- LM instruments open session
Results Obtained By Workshop Participants (PDF 876kB)
Closing Discussion
- Technology transition topics discussion session
- Closing remarks
Global Forensic and Justice Center
11200 SW 8th Street
Miami, FL 33199