Day 1

Welcome and introductions

8:30 a.m.–9:15 a.m.

  1. Purpose of the workshop
  2. Instructors
  3. Facilities
  4. Safety and laboratory protocols
  5. Agenda review
  6. Brief overview of laser microdissection (LM) and fluorescent in situ hybridization (FISH) processes

Presentation (PDF 484kB)

Lecture: Introduction to LM (Group 1)

9:15 a.m.–10:15 a.m.

  1. Applications
    • Mixtures of cells with same morphology
    • Mixtures of cells with different morphology
  2. Instrumentation
    • Laser microdissection (LM)
    • Laser capture microdissection (LCM)

Presentation (PDF 836kB)

In-Lab Training (Group 2)

9:15 a.m.–10:15 a.m.

  1. FISH slide preparation
  2. Cell mixture slide preparation

Break


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Lecture: Introduction to FISH (Group 1)

10:30 a.m.–12:30 p.m.

  1. General overview
  2. Procedure
    • CEP probes
  3. Downstream processing
    • Laser microdissection
    • Extraction
    • Amplification
  4. Applications
    • Advantages / limitations

Presentation (PDF 760kB)

In-Lab Training (Group 2)

10:30 a.m.–12:30 p.m.

  1. FISH pretreatment
  2. FISH hybridization
  3. Introduction to the Carl Zeiss PALM System

Lunch


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In-Lab Training (Group 1)

1:30 p.m.–2:30 p.m.

  1. FISH slide preparation
  2. Cell mixture slide preparation

Lecture: Introduction to LM (Group 2)

1:30 p.m.–2:30 p.m.

  1. Applications
    • Mixtures of cells with same morphology
    • Mixtures of cells with different morphology
  2. Instrumentation
    • Laser microdissection (LM)
    • Laser capture microdissection (LCM)

Break


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In-Lab Training (Group 1)

2:45 p.m.–4:45 p.m.

  1. FISH pretreatment
  2. FISH hybridization
  3. Introduction to the Carl Zeiss PALM System

Lecture: Introduction to FISH (Group 2)

2:45 p.m.–4:45 p.m.

  1. General overview
  2. Procedure
    • CEP probes
  3. Downstream processing
    • Laser microdissection
    • Extraction
    • Amplification
  4. Applications
    • Advantages / limitations


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