Laser Microdissection Workshop for Technical Managers & Supervisors Outline– Day 2
Review DNA Data from LMD samples Review DNA Data from LMD samples Review DNA Data from LMD samples
Time
Topic
Instructor(s)
8:30 – 8:40 a.m.
  1. Q&A
  2. Forensic LMD Literature
 Sanders
8:40 – 9:15 a.m. LMD Research Studies from RFUMS cont…
  1. Mixture Study
  2. Low Copy Number Study
  3. Comparative Study with Preferential Lysis
  4. Case Studies
Flash | Quicktime | WMV		 Sanders
9:15 – 9:45 a.m. Advanced LMD Forensic Applications
  1. Fluorescent Sperm Identification
  2. Chromosome Paint for Diploid Cells
  3. Validation Issues
  4. LMD on Sexual Assault Kits
Flash | Quicktime | WMV		 Wojtkiewicz
9:45 – 10:00 a.m. Break  
10:00 – 11:00 a.m. LMD LAB
  1. Fluorescence
  2. AVC Software Module
  3. Advanced Operations
Flash | Quicktime | WMV		 Wojtkiewicz
Lee
11:00 – 11:45 a.m. LMD Microscopy Research and Experience from NLCL
  1. Why LMD for NLCL Casework
  2. Elimination of Manual Extraction
  3. Reproducibility
  4. Sensitivity
  5. Troubleshooting
  6. Single Amplification/Optimization
  7. Absence of Sperm
  8. LMD: Streamlined, Novel Process
  9. Casework Examples
Flash | Quicktime | WMV		 Raley
11:45 a.m. – 12:45 p.m. Lunch  
12:45 – 1:30 p.m. Research on Chromosome Paint of Diploid Cells
  1. Cell Preparation for Chromosome Paint
  2. Male/Female Diploid Cells Mixtures
  3. Non-probative case results
 Wojtkiewicz
  Quick Break  
1:30 – 2:15 p.m. Novel Processing Plan for the Implementation of LMD Analysis of Sexual Assault Evidence.

Flash | Quicktime | WMV		 Wojtkiewicz
2:15 – 2:30 p.m. Break  
2:30 – 3:00 p.m.Review DNA Data from LMD samples

Flash | Quicktime | WMV		 Sanders
3:00 – 4:00 p.m.Open Discussion Topics concerning LMD
  1. Admissibility / voir dire issues
  2. QA & accreditation
  3. Competency training
  4. Harmonious implementation
  5. Commitments within the forensic community
 All Participants
4:00 – 4:30 p.m.Q&A and Closing  

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