Laser Microdissection Workshop for Technical Managers && Supervisors Agenda – Day 1 |
Time |
Topic |
Instructor(s) |
8:30 – 9:00 a.m. | Welcome and Introduction
Watch Video |
Sanders, Wojtkiewicz, Raley |
9:00 – 10:00 a.m. | Introduction to LMD technology
|
Wojtkiewicz |
10:00 – 10:15 a.m. | Break | |
10:15 – 10:30 a.m. | Basic cell identification: sperm and epithelial cells Watch Video |
Raley |
10:30 – 10:45 a.m. | LMD dissection of sperm cells from a mixture Watch Video |
Sanders |
10:45 – 11:00 a.m. | Lab Practical Overview Watch Video |
Sanders |
11:00 – 11:15 a.m. | LMD LAB – Care and Basics of LMD 6000 | Lee |
11:15 a.m. – 12:00 p.m. | LMD LAB - Demo of Leica LMD 6000: Using the microscope | Sanders Raley |
12:00 – 1:00 p.m. | Lunch | |
1:00 – 2:00 p.m. | LMD LAB - LMD exercises – Recovery of cell samples Groups 1 && 2 |
Sanders Raley |
WET/CHEM LAB - Fluorescence Sample Prep. - Groups 3 && 4 |
Wojtkiewicz | |
1:00 – 2:00 p.m. | LMD LAB - LMD exercises – Recovery of cell samples Groups 3 && 4 |
Sanders Raley |
WET/CHEM LAB - Fluorescence Sample Prep. - Groups 1 && 2 |
Wojtkiewicz | |
3:00 – 3:15 p.m. | Break | |
3:15 – 3:30 p.m. | Review of protocols performed by NFSTC Staff | Sanders |
3:30 – 4:20 p.m. | LMD Research Studies from RFUMS
Watch Video |
Sanders |
4:20 – 4:30 p.m. | Q&A |
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