- 01 Introduction
- 02 Biology of DNA
- 03 Practical Issues Specific to DNA Evidence
- 04 Introduction to the Forensic DNA Laboratory
- 05 Assuring Quality in DNA Testing
- 06 Understanding a Forensic DNA Lab Report
- 07 Statistics and Population Genetics
- 08 Mitochondrial DNA & Y-STR Analysis
- 09 Forensic DNA Databases
- 10 Collection of DNA Evidence from Suspects and Arrestees
- 11 Pretrial DNA Evidence Issues
- 12 Victim Issues
- 13 Trial Presentation
- 14 Postconviction DNA Cases
- 15 Emerging Trends
Principles of Forensic DNA
for Officers of the Court
for Officers of the Court
Module 8 :: mtDNA & Y-STR
Topic 2 :: mtDNA Laboratory Analysis
Sequencing
The third step in mtDNA analysis is different:
- Sequencing: Dideoxy sequencing, a well-established technique that originated in 1977, places fluorescent chemical tags on the terminal nucleotides of DNA fragments that differ in length by a single nucleotide. When these fragments are electrophoresed through a sequence detection instrument, a laser excites each fluorescent tag as it passes by. A camera detects the emitted fluorescence and "reads" the sequence or order of the DNA nucleotides
